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non human related sirnas  (Santa Cruz Biotechnology)


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    Santa Cruz Biotechnology non human related sirnas
    (A) mRNA expression analysis was performed for the A427 and A549 lung adenocarcinoma cell lines in the presence or absence of cisplatinum-based treatment, and the effects of negative control scramble <t>siRNAs</t> and specific anti-MEOX2 siRNAs were assessed. The results are shown for two representative biological experiments performed in triplicate, with * p ≤0.05, and ** p ≤0.01. (B) A427 and A549 lung adenocarcinoma cells exhibited a cisplatinum-inducible GLI-1 protein expression pattern at IC:12.5 (8 μM), while reduced inducible GLI-1 expression was observed following transfection with anti-MEOX2 siRNA in the presence of 8 μM cisplatinum. Western blot statistical analyses, assessed via quantitative densitometry, were performed to determine * p ≤0.05 and ** p ≤0.005 using Student's t -test as well as one-way ANOVA with Dunnett's and Tukey's multiple comparison tests. Western blot bands were quantified as the pixel total intensity rate and expressed as a Change Index normalized to GAPDH. Images are representative of 3 biological replicates. Quantification analyses were performed using cisplatinum (0 μM) treatment as a negative control reference. Images were obtained using a C-DIGIT device (LICOR). Pixel quantification and data analyses were carried out using Image Studio software; the total pixel intensity for each specific protein product was normalized to GAPDH.
    Non Human Related Sirnas, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 178 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/non+human+related+sirnas/Control+siRNA-B/pmc05620156-243-1-6
    Average 93 stars, based on 178 article reviews
    non human related sirnas - by Bioz Stars, 2026-09
    93/100 stars

    Images

    1) Product Images from "Epigenomic study identifies a novel mesenchyme homeobox2-GLI1 transcription axis involved in cancer drug resistance, overall survival and therapy prognosis in lung cancer patients"

    Article Title: Epigenomic study identifies a novel mesenchyme homeobox2-GLI1 transcription axis involved in cancer drug resistance, overall survival and therapy prognosis in lung cancer patients

    Journal: Oncotarget

    doi: 10.18632/oncotarget.17715

    (A) mRNA expression analysis was performed for the A427 and A549 lung adenocarcinoma cell lines in the presence or absence of cisplatinum-based treatment, and the effects of negative control scramble siRNAs and specific anti-MEOX2 siRNAs were assessed. The results are shown for two representative biological experiments performed in triplicate, with * p ≤0.05, and ** p ≤0.01. (B) A427 and A549 lung adenocarcinoma cells exhibited a cisplatinum-inducible GLI-1 protein expression pattern at IC:12.5 (8 μM), while reduced inducible GLI-1 expression was observed following transfection with anti-MEOX2 siRNA in the presence of 8 μM cisplatinum. Western blot statistical analyses, assessed via quantitative densitometry, were performed to determine * p ≤0.05 and ** p ≤0.005 using Student's t -test as well as one-way ANOVA with Dunnett's and Tukey's multiple comparison tests. Western blot bands were quantified as the pixel total intensity rate and expressed as a Change Index normalized to GAPDH. Images are representative of 3 biological replicates. Quantification analyses were performed using cisplatinum (0 μM) treatment as a negative control reference. Images were obtained using a C-DIGIT device (LICOR). Pixel quantification and data analyses were carried out using Image Studio software; the total pixel intensity for each specific protein product was normalized to GAPDH.
    Figure Legend Snippet: (A) mRNA expression analysis was performed for the A427 and A549 lung adenocarcinoma cell lines in the presence or absence of cisplatinum-based treatment, and the effects of negative control scramble siRNAs and specific anti-MEOX2 siRNAs were assessed. The results are shown for two representative biological experiments performed in triplicate, with * p ≤0.05, and ** p ≤0.01. (B) A427 and A549 lung adenocarcinoma cells exhibited a cisplatinum-inducible GLI-1 protein expression pattern at IC:12.5 (8 μM), while reduced inducible GLI-1 expression was observed following transfection with anti-MEOX2 siRNA in the presence of 8 μM cisplatinum. Western blot statistical analyses, assessed via quantitative densitometry, were performed to determine * p ≤0.05 and ** p ≤0.005 using Student's t -test as well as one-way ANOVA with Dunnett's and Tukey's multiple comparison tests. Western blot bands were quantified as the pixel total intensity rate and expressed as a Change Index normalized to GAPDH. Images are representative of 3 biological replicates. Quantification analyses were performed using cisplatinum (0 μM) treatment as a negative control reference. Images were obtained using a C-DIGIT device (LICOR). Pixel quantification and data analyses were carried out using Image Studio software; the total pixel intensity for each specific protein product was normalized to GAPDH.

    Techniques Used: Expressing, Negative Control, Transfection, Western Blot, Comparison, Software

    (A) A549 and H1975 lung cancer cells demonstrated an inducible GLI-1 protein expression pattern following treatment with 8 μM cisplatinum and reduced GLI-1 inducible expression following the application of specific anti-MEOX2 siRNA and/or anti-MEOX2 siRNA plus anti-GLI1 siRNA in the presence of 8 μM cisplatinum. Western blot statistical analyses, assessed via quantitative densitometry, were performed to determine * p ≤0.05 by one-way ANOVA and Dunnett's test for multiple comparisons to identify significant differences with respect to controls. Student's t -test was performed to identify significant differences between control and cisplatinum treatment. Quantification analyses were normalized to scrambled siRNA as a negative control for gene silencing. Images were obtained using a C-DIGIT device (LICOR), and pixel quantification and data analyses were carried out using Image Studio software. Total pixel intensity for each specific protein product was normalized to GAPDH. (B) Cell culture images and graphs showing the quantitative analysis of cellular migration as a percentage (transwell migration assays) indicated significant MEOX2 and GLI-1 protein-dependent functions following the individual and combined application of anti-MEOX2 and anti-GLI1 siRNAs in A549, NH2347 and H1975 lung adenocarcinoma cells; ** p ≤0.005 and *** p ≤0.0001 based on one-way ANOVA and Dunnett's multiple comparisons test. (C) Cell culture images and graphs showing the quantitative analysis of cellular proliferation (clonogenic assays) indicated significant MEOX2 and GLI-1 protein-dependent functions following the individual and combined application of anti-MEOX2 and anti-GLI1 siRNAs in A549, NH2347 and H1975 lung adenocarcinoma cells; ** p ≤0.005 and *** p ≤0.0001 based on one-way ANOVA and Dunnett's multiple comparisons test. Transwell migration index and colony growth (clonogenic assays) rates were normalized and quantified using the ImageJ Colony Number plugin tool (see Materials and Methods).
    Figure Legend Snippet: (A) A549 and H1975 lung cancer cells demonstrated an inducible GLI-1 protein expression pattern following treatment with 8 μM cisplatinum and reduced GLI-1 inducible expression following the application of specific anti-MEOX2 siRNA and/or anti-MEOX2 siRNA plus anti-GLI1 siRNA in the presence of 8 μM cisplatinum. Western blot statistical analyses, assessed via quantitative densitometry, were performed to determine * p ≤0.05 by one-way ANOVA and Dunnett's test for multiple comparisons to identify significant differences with respect to controls. Student's t -test was performed to identify significant differences between control and cisplatinum treatment. Quantification analyses were normalized to scrambled siRNA as a negative control for gene silencing. Images were obtained using a C-DIGIT device (LICOR), and pixel quantification and data analyses were carried out using Image Studio software. Total pixel intensity for each specific protein product was normalized to GAPDH. (B) Cell culture images and graphs showing the quantitative analysis of cellular migration as a percentage (transwell migration assays) indicated significant MEOX2 and GLI-1 protein-dependent functions following the individual and combined application of anti-MEOX2 and anti-GLI1 siRNAs in A549, NH2347 and H1975 lung adenocarcinoma cells; ** p ≤0.005 and *** p ≤0.0001 based on one-way ANOVA and Dunnett's multiple comparisons test. (C) Cell culture images and graphs showing the quantitative analysis of cellular proliferation (clonogenic assays) indicated significant MEOX2 and GLI-1 protein-dependent functions following the individual and combined application of anti-MEOX2 and anti-GLI1 siRNAs in A549, NH2347 and H1975 lung adenocarcinoma cells; ** p ≤0.005 and *** p ≤0.0001 based on one-way ANOVA and Dunnett's multiple comparisons test. Transwell migration index and colony growth (clonogenic assays) rates were normalized and quantified using the ImageJ Colony Number plugin tool (see Materials and Methods).

    Techniques Used: Expressing, Western Blot, Control, Negative Control, Software, Cell Culture, Migration

    Related Articles

    Expressing:

    Article Title: Epigenomic study identifies a novel mesenchyme homeobox2-GLI1 transcription axis involved in cancer drug resistance, overall survival and therapy prognosis in lung cancer patients
    Article Snippet: We used the anti-MEOX2 (sc-106233), and GLI-1 (sc-37911) siRNAs, each consisting of a pool (cocktail) of 3 target-specific siRNAs (19-25 nt) designed to knock down corresponding human gene expression.We used the anti-MEOX2 (sc-106233), and GLI-1 (sc-37911) siRNAs, each consisting of a pool (cocktail) of 3 target-specific siRNAs (19-25 nt) designed to knock down corresponding human gene expression.. Two non-human related siRNAs obtained from Santa Cruz Biotechnology (Dallas, TX, USA) were used as negative controls (sc-37007 and sc-44230).. Briefly, 3×10 5 cells in RPMI-1640 (Biowest, USA) and 3% antibiotic-free FBS (Biowest, USA) were incubated in 6-well plates for 12 hours and then transfected for a total of 48 hours with Lipofectamine 2000® (Invitrogen, Foster City, CA, USA) and siRNAs at 50 nM in the presence or absence of a cisplatinum drug in a total volume of 2,500 μl.Briefly, 3×10 5 cells in RPMI-1640 (Biowest, USA) and 3% antibiotic-free FBS (Biowest, USA) were incubated in 6-well plates for 12 hours and then transfected for a total of 48 hours with Lipofectamine 2000® (Invitrogen, Foster City, CA, USA) and siRNAs at 50 nM in the presence or absence of a cisplatinum drug in a total volume..

    Negative Control:

    Article Title: Epigenomic study identifies a novel mesenchyme homeobox2-GLI1 transcription axis involved in cancer drug resistance, overall survival and therapy prognosis in lung cancer patients
    Article Snippet: We used the anti-MEOX2 (sc-106233), and GLI-1 (sc-37911) siRNAs, each consisting of a pool (cocktail) of 3 target-specific siRNAs (19-25 nt) designed to knock down corresponding human gene expression.We used the anti-MEOX2 (sc-106233), and GLI-1 (sc-37911) siRNAs, each consisting of a pool (cocktail) of 3 target-specific siRNAs (19-25 nt) designed to knock down corresponding human gene expression.. Two non-human related siRNAs obtained from Santa Cruz Biotechnology (Dallas, TX, USA) were used as negative controls (sc-37007 and sc-44230).. Briefly, 3×10 5 cells in RPMI-1640 (Biowest, USA) and 3% antibiotic-free FBS (Biowest, USA) were incubated in 6-well plates for 12 hours and then transfected for a total of 48 hours with Lipofectamine 2000® (Invitrogen, Foster City, CA, USA) and siRNAs at 50 nM in the presence or absence of a cisplatinum drug in a total volume of 2,500 μl.Briefly, 3×10 5 cells in RPMI-1640 (Biowest, USA) and 3% antibiotic-free FBS (Biowest, USA) were incubated in 6-well plates for 12 hours and then transfected for a total of 48 hours with Lipofectamine 2000® (Invitrogen, Foster City, CA, USA) and siRNAs at 50 nM in the presence or absence of a cisplatinum drug in a total volume..

    Transfection:

    Article Title: Epigenomic study identifies a novel mesenchyme homeobox2-GLI1 transcription axis involved in cancer drug resistance, overall survival and therapy prognosis in lung cancer patients
    Article Snippet: We used the anti-MEOX2 (sc-106233), and GLI-1 (sc-37911) siRNAs, each consisting of a pool (cocktail) of 3 target-specific siRNAs (19-25 nt) designed to knock down corresponding human gene expression.We used the anti-MEOX2 (sc-106233), and GLI-1 (sc-37911) siRNAs, each consisting of a pool (cocktail) of 3 target-specific siRNAs (19-25 nt) designed to knock down corresponding human gene expression.. Two non-human related siRNAs obtained from Santa Cruz Biotechnology (Dallas, TX, USA) were used as negative controls (sc-37007 and sc-44230).. Briefly, 3×10 5 cells in RPMI-1640 (Biowest, USA) and 3% antibiotic-free FBS (Biowest, USA) were incubated in 6-well plates for 12 hours and then transfected for a total of 48 hours with Lipofectamine 2000® (Invitrogen, Foster City, CA, USA) and siRNAs at 50 nM in the presence or absence of a cisplatinum drug in a total volume of 2,500 μl.Briefly, 3×10 5 cells in RPMI-1640 (Biowest, USA) and 3% antibiotic-free FBS (Biowest, USA) were incubated in 6-well plates for 12 hours and then transfected for a total of 48 hours with Lipofectamine 2000® (Invitrogen, Foster City, CA, USA) and siRNAs at 50 nM in the presence or absence of a cisplatinum drug in a total volume..

    Western Blot:

    Article Title: Epigenomic study identifies a novel mesenchyme homeobox2-GLI1 transcription axis involved in cancer drug resistance, overall survival and therapy prognosis in lung cancer patients
    Article Snippet: We used the anti-MEOX2 (sc-106233), and GLI-1 (sc-37911) siRNAs, each consisting of a pool (cocktail) of 3 target-specific siRNAs (19-25 nt) designed to knock down corresponding human gene expression.We used the anti-MEOX2 (sc-106233), and GLI-1 (sc-37911) siRNAs, each consisting of a pool (cocktail) of 3 target-specific siRNAs (19-25 nt) designed to knock down corresponding human gene expression.. Two non-human related siRNAs obtained from Santa Cruz Biotechnology (Dallas, TX, USA) were used as negative controls (sc-37007 and sc-44230).. Briefly, 3×10 5 cells in RPMI-1640 (Biowest, USA) and 3% antibiotic-free FBS (Biowest, USA) were incubated in 6-well plates for 12 hours and then transfected for a total of 48 hours with Lipofectamine 2000® (Invitrogen, Foster City, CA, USA) and siRNAs at 50 nM in the presence or absence of a cisplatinum drug in a total volume of 2,500 μl.Briefly, 3×10 5 cells in RPMI-1640 (Biowest, USA) and 3% antibiotic-free FBS (Biowest, USA) were incubated in 6-well plates for 12 hours and then transfected for a total of 48 hours with Lipofectamine 2000® (Invitrogen, Foster City, CA, USA) and siRNAs at 50 nM in the presence or absence of a cisplatinum drug in a total volume..

    Comparison:

    Article Title: Epigenomic study identifies a novel mesenchyme homeobox2-GLI1 transcription axis involved in cancer drug resistance, overall survival and therapy prognosis in lung cancer patients
    Article Snippet: We used the anti-MEOX2 (sc-106233), and GLI-1 (sc-37911) siRNAs, each consisting of a pool (cocktail) of 3 target-specific siRNAs (19-25 nt) designed to knock down corresponding human gene expression.We used the anti-MEOX2 (sc-106233), and GLI-1 (sc-37911) siRNAs, each consisting of a pool (cocktail) of 3 target-specific siRNAs (19-25 nt) designed to knock down corresponding human gene expression.. Two non-human related siRNAs obtained from Santa Cruz Biotechnology (Dallas, TX, USA) were used as negative controls (sc-37007 and sc-44230).. Briefly, 3×10 5 cells in RPMI-1640 (Biowest, USA) and 3% antibiotic-free FBS (Biowest, USA) were incubated in 6-well plates for 12 hours and then transfected for a total of 48 hours with Lipofectamine 2000® (Invitrogen, Foster City, CA, USA) and siRNAs at 50 nM in the presence or absence of a cisplatinum drug in a total volume of 2,500 μl.Briefly, 3×10 5 cells in RPMI-1640 (Biowest, USA) and 3% antibiotic-free FBS (Biowest, USA) were incubated in 6-well plates for 12 hours and then transfected for a total of 48 hours with Lipofectamine 2000® (Invitrogen, Foster City, CA, USA) and siRNAs at 50 nM in the presence or absence of a cisplatinum drug in a total volume..

    Software:

    Article Title: Epigenomic study identifies a novel mesenchyme homeobox2-GLI1 transcription axis involved in cancer drug resistance, overall survival and therapy prognosis in lung cancer patients
    Article Snippet: We used the anti-MEOX2 (sc-106233), and GLI-1 (sc-37911) siRNAs, each consisting of a pool (cocktail) of 3 target-specific siRNAs (19-25 nt) designed to knock down corresponding human gene expression.We used the anti-MEOX2 (sc-106233), and GLI-1 (sc-37911) siRNAs, each consisting of a pool (cocktail) of 3 target-specific siRNAs (19-25 nt) designed to knock down corresponding human gene expression.. Two non-human related siRNAs obtained from Santa Cruz Biotechnology (Dallas, TX, USA) were used as negative controls (sc-37007 and sc-44230).. Briefly, 3×10 5 cells in RPMI-1640 (Biowest, USA) and 3% antibiotic-free FBS (Biowest, USA) were incubated in 6-well plates for 12 hours and then transfected for a total of 48 hours with Lipofectamine 2000® (Invitrogen, Foster City, CA, USA) and siRNAs at 50 nM in the presence or absence of a cisplatinum drug in a total volume of 2,500 μl.Briefly, 3×10 5 cells in RPMI-1640 (Biowest, USA) and 3% antibiotic-free FBS (Biowest, USA) were incubated in 6-well plates for 12 hours and then transfected for a total of 48 hours with Lipofectamine 2000® (Invitrogen, Foster City, CA, USA) and siRNAs at 50 nM in the presence or absence of a cisplatinum drug in a total volume..

    Control:

    Article Title: Epigenomic study identifies a novel mesenchyme homeobox2-GLI1 transcription axis involved in cancer drug resistance, overall survival and therapy prognosis in lung cancer patients
    Article Snippet: We used the anti-MEOX2 (sc-106233), and GLI-1 (sc-37911) siRNAs, each consisting of a pool (cocktail) of 3 target-specific siRNAs (19-25 nt) designed to knock down corresponding human gene expression.We used the anti-MEOX2 (sc-106233), and GLI-1 (sc-37911) siRNAs, each consisting of a pool (cocktail) of 3 target-specific siRNAs (19-25 nt) designed to knock down corresponding human gene expression.. Two non-human related siRNAs obtained from Santa Cruz Biotechnology (Dallas, TX, USA) were used as negative controls (sc-37007 and sc-44230).. Briefly, 3×10 5 cells in RPMI-1640 (Biowest, USA) and 3% antibiotic-free FBS (Biowest, USA) were incubated in 6-well plates for 12 hours and then transfected for a total of 48 hours with Lipofectamine 2000® (Invitrogen, Foster City, CA, USA) and siRNAs at 50 nM in the presence or absence of a cisplatinum drug in a total volume of 2,500 μl.Briefly, 3×10 5 cells in RPMI-1640 (Biowest, USA) and 3% antibiotic-free FBS (Biowest, USA) were incubated in 6-well plates for 12 hours and then transfected for a total of 48 hours with Lipofectamine 2000® (Invitrogen, Foster City, CA, USA) and siRNAs at 50 nM in the presence or absence of a cisplatinum drug in a total volume..

    Cell Culture:

    Article Title: Epigenomic study identifies a novel mesenchyme homeobox2-GLI1 transcription axis involved in cancer drug resistance, overall survival and therapy prognosis in lung cancer patients
    Article Snippet: We used the anti-MEOX2 (sc-106233), and GLI-1 (sc-37911) siRNAs, each consisting of a pool (cocktail) of 3 target-specific siRNAs (19-25 nt) designed to knock down corresponding human gene expression.We used the anti-MEOX2 (sc-106233), and GLI-1 (sc-37911) siRNAs, each consisting of a pool (cocktail) of 3 target-specific siRNAs (19-25 nt) designed to knock down corresponding human gene expression.. Two non-human related siRNAs obtained from Santa Cruz Biotechnology (Dallas, TX, USA) were used as negative controls (sc-37007 and sc-44230).. Briefly, 3×10 5 cells in RPMI-1640 (Biowest, USA) and 3% antibiotic-free FBS (Biowest, USA) were incubated in 6-well plates for 12 hours and then transfected for a total of 48 hours with Lipofectamine 2000® (Invitrogen, Foster City, CA, USA) and siRNAs at 50 nM in the presence or absence of a cisplatinum drug in a total volume of 2,500 μl.Briefly, 3×10 5 cells in RPMI-1640 (Biowest, USA) and 3% antibiotic-free FBS (Biowest, USA) were incubated in 6-well plates for 12 hours and then transfected for a total of 48 hours with Lipofectamine 2000® (Invitrogen, Foster City, CA, USA) and siRNAs at 50 nM in the presence or absence of a cisplatinum drug in a total volume..

    Migration:

    Article Title: Epigenomic study identifies a novel mesenchyme homeobox2-GLI1 transcription axis involved in cancer drug resistance, overall survival and therapy prognosis in lung cancer patients
    Article Snippet: We used the anti-MEOX2 (sc-106233), and GLI-1 (sc-37911) siRNAs, each consisting of a pool (cocktail) of 3 target-specific siRNAs (19-25 nt) designed to knock down corresponding human gene expression.We used the anti-MEOX2 (sc-106233), and GLI-1 (sc-37911) siRNAs, each consisting of a pool (cocktail) of 3 target-specific siRNAs (19-25 nt) designed to knock down corresponding human gene expression.. Two non-human related siRNAs obtained from Santa Cruz Biotechnology (Dallas, TX, USA) were used as negative controls (sc-37007 and sc-44230).. Briefly, 3×10 5 cells in RPMI-1640 (Biowest, USA) and 3% antibiotic-free FBS (Biowest, USA) were incubated in 6-well plates for 12 hours and then transfected for a total of 48 hours with Lipofectamine 2000® (Invitrogen, Foster City, CA, USA) and siRNAs at 50 nM in the presence or absence of a cisplatinum drug in a total volume of 2,500 μl.Briefly, 3×10 5 cells in RPMI-1640 (Biowest, USA) and 3% antibiotic-free FBS (Biowest, USA) were incubated in 6-well plates for 12 hours and then transfected for a total of 48 hours with Lipofectamine 2000® (Invitrogen, Foster City, CA, USA) and siRNAs at 50 nM in the presence or absence of a cisplatinum drug in a total volume..



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    non human related sirnas  (Santa Cruz Biotechnology)
    93
    Santa Cruz Biotechnology non human related sirnas
    (A) mRNA expression analysis was performed for the A427 and A549 lung adenocarcinoma cell lines in the presence or absence of cisplatinum-based treatment, and the effects of negative control scramble <t>siRNAs</t> and specific anti-MEOX2 siRNAs were assessed. The results are shown for two representative biological experiments performed in triplicate, with * p ≤0.05, and ** p ≤0.01. (B) A427 and A549 lung adenocarcinoma cells exhibited a cisplatinum-inducible GLI-1 protein expression pattern at IC:12.5 (8 μM), while reduced inducible GLI-1 expression was observed following transfection with anti-MEOX2 siRNA in the presence of 8 μM cisplatinum. Western blot statistical analyses, assessed via quantitative densitometry, were performed to determine * p ≤0.05 and ** p ≤0.005 using Student's t -test as well as one-way ANOVA with Dunnett's and Tukey's multiple comparison tests. Western blot bands were quantified as the pixel total intensity rate and expressed as a Change Index normalized to GAPDH. Images are representative of 3 biological replicates. Quantification analyses were performed using cisplatinum (0 μM) treatment as a negative control reference. Images were obtained using a C-DIGIT device (LICOR). Pixel quantification and data analyses were carried out using Image Studio software; the total pixel intensity for each specific protein product was normalized to GAPDH.
    Non Human Related Sirnas, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/non+human+related+sirnas/Control+siRNA-B/pmc05620156-243-1-6
    Average 93 stars, based on 1 article reviews
    non human related sirnas - by Bioz Stars, 2026-09
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    non human related mrna  (Santa Cruz Biotechnology)
    96
    Santa Cruz Biotechnology non human related mrna
    (A) MEOX2 , HDAC9 and TWIST1 <t>mRNA</t> expression. Error bars represent 95% of confidence interval of the mean. (B) Protein expression in adjacent non-involved lung tissue (LNAT), lung precursor lesions (LP) and lung carcinomas (LT) (microphotographs at 200X and 400X), as well as formalin-fixed and paraffin-embedded (FFPE) and fresh frozen (FF) tissues, were compared. (C) Promoter sequence methylation analysis. The differences were statistically significant with respect to the LNAT and were detected using Fisher's exact test, an unpaired t -test and a Mann-Whitney U test, * p ≤0.05; ** p ≤0.005; *** p ≤0.001. Error bars indicating min to max rank with 95% confidence interval, and box plots represent standard deviations of the mean.
    Non Human Related Mrna, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    (A) mRNA expression analysis was performed for the A427 and A549 lung adenocarcinoma cell lines in the presence or absence of cisplatinum-based treatment, and the effects of negative control scramble siRNAs and specific anti-MEOX2 siRNAs were assessed. The results are shown for two representative biological experiments performed in triplicate, with * p ≤0.05, and ** p ≤0.01. (B) A427 and A549 lung adenocarcinoma cells exhibited a cisplatinum-inducible GLI-1 protein expression pattern at IC:12.5 (8 μM), while reduced inducible GLI-1 expression was observed following transfection with anti-MEOX2 siRNA in the presence of 8 μM cisplatinum. Western blot statistical analyses, assessed via quantitative densitometry, were performed to determine * p ≤0.05 and ** p ≤0.005 using Student's t -test as well as one-way ANOVA with Dunnett's and Tukey's multiple comparison tests. Western blot bands were quantified as the pixel total intensity rate and expressed as a Change Index normalized to GAPDH. Images are representative of 3 biological replicates. Quantification analyses were performed using cisplatinum (0 μM) treatment as a negative control reference. Images were obtained using a C-DIGIT device (LICOR). Pixel quantification and data analyses were carried out using Image Studio software; the total pixel intensity for each specific protein product was normalized to GAPDH.

    Journal: Oncotarget

    Article Title: Epigenomic study identifies a novel mesenchyme homeobox2-GLI1 transcription axis involved in cancer drug resistance, overall survival and therapy prognosis in lung cancer patients

    doi: 10.18632/oncotarget.17715

    Figure Lengend Snippet: (A) mRNA expression analysis was performed for the A427 and A549 lung adenocarcinoma cell lines in the presence or absence of cisplatinum-based treatment, and the effects of negative control scramble siRNAs and specific anti-MEOX2 siRNAs were assessed. The results are shown for two representative biological experiments performed in triplicate, with * p ≤0.05, and ** p ≤0.01. (B) A427 and A549 lung adenocarcinoma cells exhibited a cisplatinum-inducible GLI-1 protein expression pattern at IC:12.5 (8 μM), while reduced inducible GLI-1 expression was observed following transfection with anti-MEOX2 siRNA in the presence of 8 μM cisplatinum. Western blot statistical analyses, assessed via quantitative densitometry, were performed to determine * p ≤0.05 and ** p ≤0.005 using Student's t -test as well as one-way ANOVA with Dunnett's and Tukey's multiple comparison tests. Western blot bands were quantified as the pixel total intensity rate and expressed as a Change Index normalized to GAPDH. Images are representative of 3 biological replicates. Quantification analyses were performed using cisplatinum (0 μM) treatment as a negative control reference. Images were obtained using a C-DIGIT device (LICOR). Pixel quantification and data analyses were carried out using Image Studio software; the total pixel intensity for each specific protein product was normalized to GAPDH.

    Article Snippet: Two non-human related siRNAs obtained from Santa Cruz Biotechnology (Dallas, TX, USA) were used as negative controls (sc-37007 and sc-44230).

    Techniques: Expressing, Negative Control, Transfection, Western Blot, Comparison, Software

    (A) A549 and H1975 lung cancer cells demonstrated an inducible GLI-1 protein expression pattern following treatment with 8 μM cisplatinum and reduced GLI-1 inducible expression following the application of specific anti-MEOX2 siRNA and/or anti-MEOX2 siRNA plus anti-GLI1 siRNA in the presence of 8 μM cisplatinum. Western blot statistical analyses, assessed via quantitative densitometry, were performed to determine * p ≤0.05 by one-way ANOVA and Dunnett's test for multiple comparisons to identify significant differences with respect to controls. Student's t -test was performed to identify significant differences between control and cisplatinum treatment. Quantification analyses were normalized to scrambled siRNA as a negative control for gene silencing. Images were obtained using a C-DIGIT device (LICOR), and pixel quantification and data analyses were carried out using Image Studio software. Total pixel intensity for each specific protein product was normalized to GAPDH. (B) Cell culture images and graphs showing the quantitative analysis of cellular migration as a percentage (transwell migration assays) indicated significant MEOX2 and GLI-1 protein-dependent functions following the individual and combined application of anti-MEOX2 and anti-GLI1 siRNAs in A549, NH2347 and H1975 lung adenocarcinoma cells; ** p ≤0.005 and *** p ≤0.0001 based on one-way ANOVA and Dunnett's multiple comparisons test. (C) Cell culture images and graphs showing the quantitative analysis of cellular proliferation (clonogenic assays) indicated significant MEOX2 and GLI-1 protein-dependent functions following the individual and combined application of anti-MEOX2 and anti-GLI1 siRNAs in A549, NH2347 and H1975 lung adenocarcinoma cells; ** p ≤0.005 and *** p ≤0.0001 based on one-way ANOVA and Dunnett's multiple comparisons test. Transwell migration index and colony growth (clonogenic assays) rates were normalized and quantified using the ImageJ Colony Number plugin tool (see Materials and Methods).

    Journal: Oncotarget

    Article Title: Epigenomic study identifies a novel mesenchyme homeobox2-GLI1 transcription axis involved in cancer drug resistance, overall survival and therapy prognosis in lung cancer patients

    doi: 10.18632/oncotarget.17715

    Figure Lengend Snippet: (A) A549 and H1975 lung cancer cells demonstrated an inducible GLI-1 protein expression pattern following treatment with 8 μM cisplatinum and reduced GLI-1 inducible expression following the application of specific anti-MEOX2 siRNA and/or anti-MEOX2 siRNA plus anti-GLI1 siRNA in the presence of 8 μM cisplatinum. Western blot statistical analyses, assessed via quantitative densitometry, were performed to determine * p ≤0.05 by one-way ANOVA and Dunnett's test for multiple comparisons to identify significant differences with respect to controls. Student's t -test was performed to identify significant differences between control and cisplatinum treatment. Quantification analyses were normalized to scrambled siRNA as a negative control for gene silencing. Images were obtained using a C-DIGIT device (LICOR), and pixel quantification and data analyses were carried out using Image Studio software. Total pixel intensity for each specific protein product was normalized to GAPDH. (B) Cell culture images and graphs showing the quantitative analysis of cellular migration as a percentage (transwell migration assays) indicated significant MEOX2 and GLI-1 protein-dependent functions following the individual and combined application of anti-MEOX2 and anti-GLI1 siRNAs in A549, NH2347 and H1975 lung adenocarcinoma cells; ** p ≤0.005 and *** p ≤0.0001 based on one-way ANOVA and Dunnett's multiple comparisons test. (C) Cell culture images and graphs showing the quantitative analysis of cellular proliferation (clonogenic assays) indicated significant MEOX2 and GLI-1 protein-dependent functions following the individual and combined application of anti-MEOX2 and anti-GLI1 siRNAs in A549, NH2347 and H1975 lung adenocarcinoma cells; ** p ≤0.005 and *** p ≤0.0001 based on one-way ANOVA and Dunnett's multiple comparisons test. Transwell migration index and colony growth (clonogenic assays) rates were normalized and quantified using the ImageJ Colony Number plugin tool (see Materials and Methods).

    Article Snippet: Two non-human related siRNAs obtained from Santa Cruz Biotechnology (Dallas, TX, USA) were used as negative controls (sc-37007 and sc-44230).

    Techniques: Expressing, Western Blot, Control, Negative Control, Software, Cell Culture, Migration

    (A) MEOX2 , HDAC9 and TWIST1 mRNA expression. Error bars represent 95% of confidence interval of the mean. (B) Protein expression in adjacent non-involved lung tissue (LNAT), lung precursor lesions (LP) and lung carcinomas (LT) (microphotographs at 200X and 400X), as well as formalin-fixed and paraffin-embedded (FFPE) and fresh frozen (FF) tissues, were compared. (C) Promoter sequence methylation analysis. The differences were statistically significant with respect to the LNAT and were detected using Fisher's exact test, an unpaired t -test and a Mann-Whitney U test, * p ≤0.05; ** p ≤0.005; *** p ≤0.001. Error bars indicating min to max rank with 95% confidence interval, and box plots represent standard deviations of the mean.

    Journal: PLoS ONE

    Article Title: Overexpression of MEOX2 and TWIST1 Is Associated with H3K27me3 Levels and Determines Lung Cancer Chemoresistance and Prognosis

    doi: 10.1371/journal.pone.0114104

    Figure Lengend Snippet: (A) MEOX2 , HDAC9 and TWIST1 mRNA expression. Error bars represent 95% of confidence interval of the mean. (B) Protein expression in adjacent non-involved lung tissue (LNAT), lung precursor lesions (LP) and lung carcinomas (LT) (microphotographs at 200X and 400X), as well as formalin-fixed and paraffin-embedded (FFPE) and fresh frozen (FF) tissues, were compared. (C) Promoter sequence methylation analysis. The differences were statistically significant with respect to the LNAT and were detected using Fisher's exact test, an unpaired t -test and a Mann-Whitney U test, * p ≤0.05; ** p ≤0.005; *** p ≤0.001. Error bars indicating min to max rank with 95% confidence interval, and box plots represent standard deviations of the mean.

    Article Snippet: We used small interfering RNAs (siRNAs) directed against MEOX2 (sc-106233), TWIST1 (sc-38604) and non-human related mRNA as a negative control (sc-37007 and sc-44230) all obtained from Santa Cruz Biotechnology (Dallas, Texas, USA).

    Techniques: Expressing, Sequencing, Methylation, MANN-WHITNEY

    (A) MEOX2 expression in NSCLC and the LNAT-derived samples, and (B) TWIST1 expression in NSCLC and the LNAT-derived samples. Error bars represent standard errors of the mean of three replicates. (C) Histone modification enrichment profile of the MEOX2 promoter sequence. (D) Histone modification enrichment profile of the TWIST1 promoter sequence. Box plots represent the mean of three replicates. The analysis revealed a correlation between low mRNA expression and H3K27me3 enrichment. (E) Survival curve analyses based on Log-Rank (Mantel Cox) and Gehan-Breslow-Wilcoxon tests of the relative expression index of MEOX2 plus TWIST1 ( p ≤0.0027).

    Journal: PLoS ONE

    Article Title: Overexpression of MEOX2 and TWIST1 Is Associated with H3K27me3 Levels and Determines Lung Cancer Chemoresistance and Prognosis

    doi: 10.1371/journal.pone.0114104

    Figure Lengend Snippet: (A) MEOX2 expression in NSCLC and the LNAT-derived samples, and (B) TWIST1 expression in NSCLC and the LNAT-derived samples. Error bars represent standard errors of the mean of three replicates. (C) Histone modification enrichment profile of the MEOX2 promoter sequence. (D) Histone modification enrichment profile of the TWIST1 promoter sequence. Box plots represent the mean of three replicates. The analysis revealed a correlation between low mRNA expression and H3K27me3 enrichment. (E) Survival curve analyses based on Log-Rank (Mantel Cox) and Gehan-Breslow-Wilcoxon tests of the relative expression index of MEOX2 plus TWIST1 ( p ≤0.0027).

    Article Snippet: We used small interfering RNAs (siRNAs) directed against MEOX2 (sc-106233), TWIST1 (sc-38604) and non-human related mRNA as a negative control (sc-37007 and sc-44230) all obtained from Santa Cruz Biotechnology (Dallas, Texas, USA).

    Techniques: Expressing, Derivative Assay, Modification, Sequencing

    (A) MEOX2 histone profile under baseline conditions. (B) MEOX2 histone modification profile changes after cisplatinum treatment. (C) TWIST1 histone profile under baseline conditions. (D) TWIST1 histone modification profile changes after cisplatinum treatment. (E) MEOX2 mRNA expression induction by cisplatinum stimulation as compared to the baseline conditions from both NSCLC cell lines. (F) TWIST1 mRNA expression induction by cisplatinum stimulation as compared to the baseline conditions from both NSCLC cell lines. (G) Chemoresistance curves evaluating cellular viability for both NSCLC cell lines. (H) MEOX2 and TWIST1 siRNA silencing assays and cellular viability analyses. Statistically significant differences with respect to the baseline control conditions as assessed using an unpaired t-test, * p ≤0.05 and ** p ≤0.001. Error bars represent standard errors of the mean of three technical replicates.

    Journal: PLoS ONE

    Article Title: Overexpression of MEOX2 and TWIST1 Is Associated with H3K27me3 Levels and Determines Lung Cancer Chemoresistance and Prognosis

    doi: 10.1371/journal.pone.0114104

    Figure Lengend Snippet: (A) MEOX2 histone profile under baseline conditions. (B) MEOX2 histone modification profile changes after cisplatinum treatment. (C) TWIST1 histone profile under baseline conditions. (D) TWIST1 histone modification profile changes after cisplatinum treatment. (E) MEOX2 mRNA expression induction by cisplatinum stimulation as compared to the baseline conditions from both NSCLC cell lines. (F) TWIST1 mRNA expression induction by cisplatinum stimulation as compared to the baseline conditions from both NSCLC cell lines. (G) Chemoresistance curves evaluating cellular viability for both NSCLC cell lines. (H) MEOX2 and TWIST1 siRNA silencing assays and cellular viability analyses. Statistically significant differences with respect to the baseline control conditions as assessed using an unpaired t-test, * p ≤0.05 and ** p ≤0.001. Error bars represent standard errors of the mean of three technical replicates.

    Article Snippet: We used small interfering RNAs (siRNAs) directed against MEOX2 (sc-106233), TWIST1 (sc-38604) and non-human related mRNA as a negative control (sc-37007 and sc-44230) all obtained from Santa Cruz Biotechnology (Dallas, Texas, USA).

    Techniques: Modification, Expressing, Control